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"Accelerate Functional Screening with ENFINIA™ Linear DNA and GeneFrontier's Cell-Free Protein Synthesis System"

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GeneFrontier’s cell-free coupled transcription/translation (TXTL) system, PUREfrex®, is an in vitro platform enabling rapid protein production from DNA templates, making it suitable for high-throughput workflows in protein engineering and synthetic biology.

A significant challenge for TXTL systems has been bottlenecks in DNA template synthesis. Traditional methods like plasmid templates require multi-week cloning and purification. PCR-derived DNA adds complexity and time.

To overcome these challenges, GeneFrontier evaluated Elegen’s ENFINIA™ Linear DNA, which is a ready-to-use, NGS-verified linear dsDNA produced with a proprietary cell-free manufacturing process. It can be delivered quickly, in as fast as 6 business days, supporting constructs up to 7 kb with high fidelity.

Download the case study to learn how  high-quality, ready-to-use linear dsDNA supports efficient transcription and robust protein expression, streamlining TXTL workflows and enabling faster screening.

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