Accelerate HPLC success with a structured, selectivity‑driven method development strategy powered by ACE® kits--delivering faster optimization, robust separations, and confidence from first screen to final method.
This guide presents a structured, selectivity‑driven approach to HPLC method development designed to accelerate optimization and improve separation quality.
It outlines a clear workflow—from defining analytical objectives to screening column chemistries, optimizing conditions, and confirming robustness.
The document highlights how Avantor® ACE® Method Development Kits enable rapid evaluation of complementary stationary phases, helping scientists identify optimal selectivity with minimal trial‑and‑error.
Practical recommendations for gradients, pH, temperature, and validation ensure methods are reliable, reproducible, and ready for transfer or QC deployment.
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